Specifications
REACTIVITY SPECIES
Hu Ms Rt
GENE ID
https://www.ncbi.nlm.nih.gov/gene/994;
*USAGE / SAFETY STATEMENT
For laboratory research and development purposes only.
PURIFICATION
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
IMMUNOGEN
Peptide sequence around phosphorylation site of serine 323 (S-P-S(p)-M-P) derived from Human CDC25B.
SPECIFICITY
The antibody detects endogenous levels of CDC25B only when phosphorylated at serine 323.
BACKGROUND
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
ALTERNAME
CDC25HU2; CDC25M2; Dual specificity phosphatase Cdc25B; EC 3.1.3.48; M-phase inducer phosphatase 2; MPIP2
FORM OF ANTIBODY
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
RECOMMENDED DILUTIONS
Western blotting: 1:500~1:3000<br/>
We’re currently in BETA - Interested in joining? Let us know and we’ll get you set up with early access.