Specifications
COUNTRY OF ORIGIN
United Kingdom
*USAGE / SAFETY STATEMENT
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
PURITY
>95% by SDS-PAGE and HPLC
APPLICATION.1
ELISA Capture, ELISPOT, Flow Cytometry, Neutralization, Immunoprecipitation, Functional Assays, CyTOF, IHC
PURIFICATION
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
FORMULATION
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
IMMUNOGEN
Purified Recombinant Mouse IL-4 (>98%)
SPECIFICITY
Anti-IL-4 In Vivo Antibody - Low Endotoxin (11B11) recognizes an epitope on Mouse IL-4
STORAGE BUFFER
ICH3001-100ml
ICH3002-100ml
ICH3003-100ml
APPLICATION NOTES
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.5-2.0 µg/ml.
ELISPOT Capture: This Low Endotoxin Functional Formulation antibody has been reported for use as the capture antibody in a sandwich ELISPOT assay. Aseptically coat plates at a concentration of 2-6 µg/ml is recommended.
Neutralization: Anti-Mouse IL-4 (clone 11B11) antibody can neutralize the bioactivity of natural or recombinant IL-4. The Low Endotoxin Functional Formulation purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of mouse IL-4 bioactivity in vivo.
Each investigator should determine their own optimal working dilution for specific applications.
Aggregation
Aggregation level ≤ 5%
Endotoxin
<0.5 EU/mg as determined by the LAL method
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