OC active protein (prokaryotic), Mouse

Cloud-Clone Corp. Wuhan

SKU / CAT#: APA471Mu01-200ug

^$1,548⁰⁰

usd

^$7.74/ug

APA471Mu01-200ug

OC active protein (prokaryotic), Mouse

Name: OC active protein (prokaryotic), Mouse
Price: 1548.00 USD
Availability: InStock

SKU / CAT#: APA471Mu01-200ug

MPN:

^$1,548⁰⁰

usd

^$7.74/ug

Available

SHIPS May 29th

Available

SHIPS May 29th

SKU

Unit size / Options

Availability

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Description

OC active protein (prokaryotic), Mouse

Shipping & Handling

Shipped In

Cold packs

Safety & Storage

Storage Temperature

Store at 2-8℃ for one month,-80℃ for 12 months

Safety Statement

Research Use Only

Regulatory & Compliance

Technical Documents

MANUAL (1)

Product Manual for OC active protein (prokaryotic), Mouse

Specifications

CLASS

Active proteins

SHIPPING NOTES

FedEx Service

COUNTRY OF ORIGIN

China

RAW MATERIAL ORIGINS

China

APPLICATION

Cell culture; Activity Assays.

USAGE

Reconstitute in PBS or others.

GENE ID

https://www.ncbi.nlm.nih.gov/gene/12096

UNIPROT ID

P86546

*USAGE / SAFETY STATEMENT

Research Use Only

PURITY

Greater than 95% by SDSPAGE

pH RANGE

7.4

CONCENTRATION

0.2mg/mL

MWCO (kDa)

40kDa

VALIDATED APPLICATION

Cell culture; Activity Assays.

SOURCE

E.coli

HOST SPECIES

E.coli

REACTIVITY SPECIES

Mouse

CROSS REACTIVITY

Mouse

Questions & Answers (0)

Citations (0)

Figure. The binding activity of OC with FUR.<br/>Osteocalcin (OC), also known as bone gamma-carboxyglutamic acid-containing protein (BGLAP), is a noncollagenous protein hormone found in bone and dentin. Osteocalcin is secreted solely by osteoblasts and thought to play a role in the body's metabolic regulation and is pro-osteoblastic, or bone-building, by nature. It is also implicated in bone mineralization and calcium ion homeostasis. Osteocalcin acts as a hormone in the body, causing beta cells in the pancreas to release more insulin, and at the same time directing fat cells to release the hormone adiponectin, which increases sensitivity to insulin. Besides, Furin (FUR) has been identified as an interactor of OC, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse OC and recombinant mouse FUR. Briefly, OC were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to FUR-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-OC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of OC and FUR was shown in Figure 1, and this effect was in a dose dependent manner.

Shipping & Handling

Shipped In

Cold packs

Safety & Storage

Storage Temperature

Store at 2-8℃ for one month,-80℃ for 12 months

Safety Statement

Research Use Only

Regulatory & Compliance

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