Applications:
Suitable for use in Immunohistochemistry, ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions:
Immunohistochemistry (Frozen, paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin embedded sections. Sodium citrate buffer, pH 6.0 is recommended.
Western Blot (Reducing conditions): Detects a band of 50-56kD that represents the nucleocapsid. Also detects specific bands at 56 and 46kD in reducing gels with canine coronavirus (CCV) as the antigen.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Store...
Applications:
Suitable for use in Immunohistochemistry, ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions:
Immunohistochemistry (Frozen, paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin embedded sections. Sodium citrate buffer, pH 6.0 is recommended.
Western Blot (Reducing conditions): Detects a band of 50-56kD that represents the nucleocapsid. Also detects specific bands at 56 and 46kD in reducing gels with canine coronavirus (CCV) as the antigen.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Store...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
4°C Do Not Freeze
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
Specifications
CLONE NUMBER
3H2460 (FIPV3-70)
CLASS
Monoclonal Antibodies
SUBCLASS
Infectious Disease Feline Infectious Peritonitis
APPLICATION
E IHC WB
*USAGE / SAFETY STATEMENT
For laboratory research and development purposes only.
PURITY
Purified by Protein A affinity chromatography from tissue culture supernatant.
PHYSICAL FORM
Supplied as a liquid in PBS, pH 7.2. Labeled with Alkaline Phosphatase.
GRADE
Affinity Purified
ANTIBODY TYPE
Mab
ISOTYPE
IgG2a
CROSS REACTIVITY
Fe Ft
IMMUNOGEN
Coronavirus cocktail
SPECIFIC ACTIVITY
Recognizes feline infectious peritonitis virus (FIPV) type 1 and 2. Specific for the nucleocapsid. Shown to react with canine coronavirus (CCV), porcine coronavirus transmissable gastroenteritis virus (TGEV) and ferret coronavirus. Some reactivity has been observed against bovine coronavirus (BCV). Exhibits negative reactivity against feline leukemia virus, feline immunodeficiency virus, feline calcivirus, feline Herpes virus, canine adenovirus (type 2), canine distemper virus, canine parvovirus and canine parainfluenza virus.
COMMODITY CODE
30021010
HOST
mouse
Questions & Answers (0)
Citations (0)
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
4°C Do Not Freeze
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
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