Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Positive Control:
H1838 HeLa cell extract:
Add an equal volume of Laemmli reducing sample buffer to 10ul of lysate. Boil for 5 minutes to reduce the preparation. Load 10-20ug of reduced lysate per lane for Western Blot.
Recommended secondary antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
Storage and Stability:
Store product at 4°C...
Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Positive Control:
H1838 HeLa cell extract:
Add an equal volume of Laemmli reducing sample buffer to 10ul of lysate. Boil for 5 minutes to reduce the preparation. Load 10-20ug of reduced lysate per lane for Western Blot.
Recommended secondary antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
Storage and Stability:
Store product at 4°C...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
4°C Do Not Freeze
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
Specifications
CLASS
Monoclonal Antibodies
SUBCLASS
Enzymes, Deacetylase
APPLICATION
IC IP WB
CLONE NUMBER
4H329 (3F3)
ACCESSION#
NP_001518
*USAGE / SAFETY STATEMENT
For laboratory research and development purposes only.
PURITY
Purified by Protein G affinity chromatography.
PHYSICAL FORM
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
GRADE
Affinity Purified
ANTIBODY TYPE
Mab
HOST SPECIES
human
ISOTYPE
IgG1
CROSS REACTIVITY
Bo Hm Hu Mo
IMMUNOGEN
Synthetic peptide, aa473-488 (CEKTDTKGTKSEQLSNP) corresponding to human HDAC2 (KLH) (Accession #NP_001518). Contains a N-terminal cysteine.
SPECIFIC ACTIVITY
Recognizes human HDAC2, Mr 60kD. Species Crossreactivity: mouse, bovine and hamster. Species sequence homology: rat.
SWISS PROT#
Q92769
COMMODITY CODE
30021010
HOST
mouse
Questions & Answers (0)
Citations (0)
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
4°C Do Not Freeze
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
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