Eukaryotic Initiation Factor 2B (eIF2B) plays a key role in the initiation of mRNA translation in eukaryotic cells by acting as a guanine nucleotide exchange factor. The activity of eIF2B is regulated by a wide array of conditions. Stress inhibits its action, while stimulation of protein synthesis results in its activation. eIF2Bε, an 82kD subunit of eIF2B, is phosphorylated at serine 539 by glycogen synthase kinase-3 (GSK-3), which leads to inhibition of its guanine nucleotide exchange activity.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions:
Western...
Eukaryotic Initiation Factor 2B (eIF2B) plays a key role in the initiation of mRNA translation in eukaryotic cells by acting as a guanine nucleotide exchange factor. The activity of eIF2B is regulated by a wide array of conditions. Stress inhibits its action, while stimulation of protein synthesis results in its activation. eIF2Bε, an 82kD subunit of eIF2B, is phosphorylated at serine 539 by glycogen synthase kinase-3 (GSK-3), which leads to inhibition of its guanine nucleotide exchange activity.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions:
Western...
Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
-20°C
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
Specifications
CLASS
Polyclonal Antibodies
SUBCLASS
Translation Initiation Factors
APPLICATION
E WB
*USAGE / SAFETY STATEMENT
For laboratory research and development purposes only.
PURITY
Purified by affinity chromatography.
PHYSICAL FORM
Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
GRADE
Affinity Purified
ANTIBODY TYPE
Pab
HOST SPECIES
rat
ISOTYPE
IgG
CROSS REACTIVITY
Hu Rt
IMMUNOGEN
Synthetic phosphopeptide derived from the region of rat eIF2Bε that contains serine 535 (corresponding to serine 539 in the human sequence).
SPECIFIC ACTIVITY
Recognizes rat eIF2Bε when phosphorylated at Ser539. Species sequence homology: human (80%).
COMMODITY CODE
30021010
HOST
rabbit
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Shipping & Handling
Shipped In
Cold Packs
Safety & Storage
Storage Temperature
-20°C
Safety Statement
For laboratory research and development purposes only.
Regulatory & Compliance
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