Specifications
SHIPPING NOTES
Blue Ice or 4ºC. All orders received in North America by 12:00 PM PST Monday to Thursday will usually be shipped out on the same day. North American customers will typically receive their orders within 1-2 business days. All shipments are F.O.B. from point of shipment, pre-paid and added to the invoice. Freight charges include but not limited to: priority service and packaging materials.Products are shipped with “cold packs” in insulated containers. There is a charge for this packaging and for the shipping costs via FedEx. Please inspect packages immediately upon receipt and inform Labscoop Customer Support of any shortage or damage within 48 hours of receipt. We can only accept returns after we have given prior authorization and issued specific shipping instructions. If a customer wishes to use their own FedEx account number please indicate this on the purchase order or during checkout.
APPLICATION
WB, IHC, ICC/IF, IP
GENE ID
https://www.ncbi.nlm.nih.gov/gene/287164
*USAGE / SAFETY STATEMENT
Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
ANTIBODY TYPE
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
REACTIVITY SPECIES
Human | Mouse | Rat | Bovine | Dog | Hamster | Guinea Pig (Cavia porcellus) | Sheep | Pig | African clawed frog (Xenopus laevis) | Mollusk | Mussel (Perna viridis)
PURIFICATION
Peptide Affinity Purified
QUALITY CERTIFICATION / COMPLIANCE
ISO 9001:2015
IMMUNOGEN
Rat PDI synthetic peptide (409-509 aa C-terminal) conjugated to KLH
SPECIFICITY
Detects ~58kDa.
STORAGE BUFFER
PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
ANTIBODY ID
https://antibodyregistry.org/search.php?q=AB_2703340
CELLUAR LOCALIZATIONS
Endoplasmic Reticulum | Endoplasmic Reticulum Lumen
SCIENTIFIC BACKGROUND
The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation via its oxidase activity and isomerization via its isomerase activity, as well as the reduction of disulphide bonds in proteins (1). Studies suggest BiP and PDI work together sequentially to increase oxidation of these proteins (2, 3). PDI has also been found to function as a chaperone to prevent the aggregation of unfolded substrates, and serves as a subunit of prolyl 4-hydroxylase and microsomal triglyceride transferase (4, 5).
PDI is an abundant 55kDa protein located primarily in the ER, however studies have also proved its presence in the cytosol (1). PDI has the ability to reside in the ER permanently due to the highly conserved KDEL sequence at its carboxy-terminus (6). It uses carboxy-terminal KDEL as a retention signal, and this appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor (7).
REFERENCES
1. NA K.S. et al. (2007) Mol Cells. 24(2): 261-7.
2. Mayer M., Kies U., Kammermeier R., and Buchner J. (2000) J Biol Chem. 275(38): 29421-5.
3. Delom F., Mallet B., Carayon P., and Lejeune P.J. (2001) J Biol Chem 276(24): 21337-42.
4. Schultz-Norton J.R., McDonald W.H., Yates J.R. and Nardulli A.M. (2006) Mol Endocrinol 20(9): 1982-95.
5. Turano C., Coppari S. Altieri F. and Ferraro (2002) J Cell Physiol 193: 154-163.
6. Janiszewski M. (2005) J. Biol Chem. 280(49): 40813- 40819.
7. Yoshimori T., et al. (1990) J Biol Chem. 265(26): 15984-90.
RESEARCH AREA
Cell Signaling | Protein Trafficking | Chaperone Proteins | Tags and Cell Markers | Organelle Markers
ALTERNATIVE NAMES
PDA2 Antibody, PDI Antibody, PDIA2 Antibody, PDIP Antibody, pancreatic protein disulfide isomerase Antibody